Table 2 Strains and plasmids used in this study
Strain or plasmid | Description | Source or reference | |
|---|---|---|---|
E. coli strain | Host background | ||
TG1 | K-12 strain | Stratagene | |
BL21(DE3)pLysS | B strain with Lon protease deficiency, contains pLysS plasmid expressing T7 lysozyme | Novagen | |
Plasmid | Expressed protein | MW* | |
EGFP/pQE30 | EGFP with his-tag at N-terminus, with extra 22 vector sequences. | 30578 | Previous study [13] |
ThiS-EGFP/pQE30 | ThiS fused to N-terminus of EGFP with his-tag at N-terminus. | 35719 | This study |
EGFP-ThiS/pQE30 | ThiS fused to C-terminus of EGFP with his-tag at N-terminus. | 36133 | Previous study [13] |
MoaD-EGFP/pQE30 | MoaD fused to N-terminus of EGFP with his-tag at N-terminus. | 37093 | This study |
EGFP-MoaD/pQE30 | MoaD fused to C-terminus of EGFP with his-tag at N-terminus. | 37506 | This study |
ThiS-A/pET28a | ThiS fused Insulin A chain with his-tag at N-terminus. | 13438 | Previous study [13] |
ThiS-B/pET28a | ThiS fused Insulin B with his-tag at N-terminus. | 14484 | Previous study [13] |
MoaD-A/pET28a | MoaD fused Insulin A chain with his-tag at N-terminus. | 14812 | This study |
MoaD-B/pET28a | MoaD fused Insulin B with his-tag at N-terminus. | 15858 | This study |
ThiS-mRNH/pVI | ThiS fused mRI with his-tag at N-terminus. | 58661 | Previous study [13] |
MoaD-mRNH/pVI | MoaD fused mRI with his-tag at N-terminus. | 60034 | This study |
ThiS-ΔRNH/pVI | ThiS fused ΔmRI with his-tag at N-terminus. | 45672 | This study |
MoaD-ΔRNH/pVI | MoaD fused ΔmRI with his-tag at N-terminus. | 47045 | This study |