Table 1 Primers used in this study

1: ThiS up

ATAagatctATGCAGATCCTGTTTAACGATC /Bgl II

primers 1 + 2: ThiS.

2: ThiS down

ATAgaattcAACCCCCTGCAATAACC /EcoR I

3: ThiS down

ATAggatccCCCTGCAATAACCTGAAAAAG /BamH I

primers 1 + 3: ThiS for fusion to N-terminus of targets

4: MoaD up

ATAagatctATTAAAGTTCTTTTTTTCGCCCAG / Bgl II

primers 4 + 5: MoaD for fusion to N-terminus of targets

5: MoaD down

ATAggatccTCCGGTTACCGGCGGG / BamH I

6: MoaD down

ATActcgagTTAACCTCCGGTTACCGGCGGG /Xho I

primers 4 + 6: MoaD for fusion to C-terminus of targets

7: A up

ATAagatctATGGGCATTGTGGAACAGTGCTGCAC /Bgl II

primers 7 + 8: insulin chain A

8: A down

ATActcgagTTAGTTGCAATAGTTTTCCAGCTG /Xho I

primers 4 + 8: MoaD fusion to A

9: B up

ATAagatctATGTTTGTGAACCAGCATCTGTG /Bgl II

primers 9 + 10: insulin chain B

10: B down

ATActcgagTTAGGTTTTCGGGGTATAAAAAAAG /Xho I

primers 4 + 10: MoaD fusion to B

11: EGFP up

ATAggatccATGGTGAGCAAGGGCGAGGAGCTG /BamH I

primers 11 + 12: EGFP

primers 11 + 6: MoaD fusion to C-terminus of EGFP

12: pEGFP-C-3’

TATGGCTGATTATGATCAGT /universal vector primer

primers 11 + 12: EGFP for fusion at C-turminus

13: EGFP down

ATActcgagTCACTTGTACAGCTCGTCCATG /Xho I

primers 11 + 13: EGFP for fusion at N-turminus

primers 1 + 13: ThiS fusion to N-terminal EGFP

primers 4 + 13: MoaD fusion to N-terminal EGFP

14: RNH up

ATAagatctATGAGTCTTGACATCCAGTGTGAGC /Bgl II

primers 14 + 15: mRI

15: RNH down

ATAgtcgacTCAGGAAATGATCCTCAGGGAAGG /Sal I

primers 1 + 15: ThiS fusion to ΔmRI

primers 7 + 15: MoaD fusion to mRI or ΔmRI