Figure 4

In vitro refolding of EGFP in fusion with MoaD. The refolding kinetics of N-terminal fusion of EGFP with MoaD (red line) and ThiS (blue line) were compared in vitro to that of EGFP without fusion (black line) in the upper panel, which represents an averaged result of the short term refolding curves from three independent experiments, with fluorescence (normalized to the respective final fluorescence recovered) plotted against time. In the lower panel, kinetics of an initial fast refolding phase, the following slow refolding phase, and the percentage of refolding at final stage (15 h) were compared to EGFP control. *p < 0.05; **p < 0.01