Figure 3

Fluorescence distribution of EGFP fusion proteins within E. coli. E. coli TG1 bearing EGFP without fusion or with C-terminal ThiS or MoaD fusion was cultured overnight at 37°C without IPTG induction. Cells on cover slide were subjected to confocal microscopy with laser excitation at 488 nm. Representative photos were shown in left panels as fluorescence images, middle panels as phase contrast images with inclusion bodies indicated by arrows, and right panels as their merged images. Scale bar in each photo represents 3.75 μm.