Table 3 Optimized conditions for starch hydrolysis in the presence of crude and partially purified amylolytic enzymes
Enzyme* | Enzyme Units (kU mg-1) | Starch** (gL-1) | Function | Time (minutes) | Temperature (°C) | pH |
|---|---|---|---|---|---|---|
Alpha Amylase (Crude) | 2.2 | 20.0 | Liquefaction | 120 | 65 | 5.0 |
Alpha Amylase (Partially Purified) | 9.2 | 20.0 | Liquefaction | 15 | 65 | 6.0 |
Amyloglucosidase (Crude) | 91.8 | 20.0 | Saccharification | 360 | 60 | 5.0 |
Amyloglucosidase (Partially Purified) | 393.0 | 20.0 | Saccharification | 90 | 60 | 5.0 |