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Table 2 Purification profile of starch hydrolyzing enzymes produced from Aspergillus fumigatus KIBGE-IB33

From: Saccharification and liquefaction of cassava starch: an alternative source for the production of bioethanol using amylolytic enzymes by double fermentation process

Steps

Total volume (ml)

Total enzyme units (kU)

Total protein (mg)

Specific activity (kU mg-1)

Fold purification

Alpha amylase

Crude

500

5500

2500

2.2

1.0

(NH4)2SO4 Precipitation

30

1950

210

9.2

4.1

Amyloglucosidase

Crude

500

229500

2500

91.8

1.0

(NH4)2SO4 Precipitation

30

82500

210

393

4.2