Fermentation performance and physiology of two strains of Saccharomyces cerevisiaeduring growth in high gravity spruce hydrolysate and spent sulphite liquor

Table 1 ATP, ADP and AMP concentrations throughout 24 h fermentation

Strain/condition	ATP			ADP			AMP			Energy charge
Fermentation time (h)	0	4	22	0	4	22	0	4	22	22
Thermosacc/SH	8.06 ± 0.08	8.02 ± 0.17	7.29 ± 0.16	0.18 ± 0.01	0.33 ± 0.06	0.15 ± 0.01	0.16 ± 0.03	0.15 ± 0.00	0.08 ± 0.01	0.98
Thermosacc/SH+	10.94 ± 0.43	11.47 ± 0.04	5.58 ± 0.45	0.55 ± 0.03	0.54 ± 0.01	0.19 ± 0.02	0.14 ± 0.03	0.07 ± 0.03	0.43 ± 0.09	0.92
Thermosacc/SSL	8.83 ± 0.25	5.39 ± 0.38	1.48 ± 0.17	4.76 ± 0.17	2.85 ± 0.55	1.10 ± 0.10	0.23 ± 0.05	0.21 ± 0.02	0.14 ± 0.00	0.75
Thermosacc/SSL+	12.86 ± 0.38	7.01 ± 0.92	1.75 ± 0.63	9.77 ± 0.08	6.40 ± 0.49	2.13 ± 0.57	0.14 ± 0.03	0.39 ± 0.07	0.38 ± 0.05	0.66
CCUG 53310/SH	7.28 ± 0.41	3.46 ± 0.63	2.11 ± 0.05	3.10 ± 0.67	2.4 ± 0.08	1.62 ± 0.19	3.21 ± 0.02	2.83 ± 0.06	2.17 ± 0.20	0.49
CCUG 53310/SH+	13.47 ± 0.24	5.64 ± 1.39	2.15 ± 0.03	6.73 ± 0.06	3.53 ± 0.54	1.67 ± 0.14	0.33 ± 0.01	4.99 ± 0.48	2.68 ± 0.29	0.46
CCUG 53310/SSL	4.95 ± 3.54	N.D.	7.42 ± 6.61	3.40 ± 1.79	N.D.	3.16 ± 2.13	0.05 ± 0.05	N.D.	0.08 ± 0.02	0.84
CCUG 53310/SSL+	5.77 ± 3.11	5.01 ± 0.42	6.26 ± 1.49	5.23 ± 1.65	3.46 ± 0.15	5.07 ± 1.81	0.08 ± 0.01	0.29 ± 0.10	1.59 ± 0.73	0.68

Concentrations are expressed in μmol (g DW)⁻¹ unless otherwise stated. SH = Spruce hydrolysate. SSL = Spent sulphite liquor. + indicates that the fermentation has been supplemented with yeast extract.
± indicates the maximum and minimum of two independent fermentations.

ISSN: 1472-6750