Figure 4

Data obtained from a pH and buffer screen using partially purified cholesterol oxidase from C. gleum . (A) pH and buffer screen using partially purified cholesterol oxidase from C. gleum at 25°C. The effect of pH and buffer composition upon initial rates of cholesterol oxidation using the HRP coupled cholesterol oxidation assay with ABTS as electron donor was investigated. All data points represent mean values ± SEM from triplicate determinations. 100% corresponds to 3.8 U/L. (A) pH and buffer screen at 25°C. (B) MOPS buffer screen using partially purified cholesterol oxidase from C. gleum at 25°C The effect of pH and molarity upon initial rates of cholesterol oxidation using the HRP coupled cholesterol oxidation assay with ABTS as electron donor was investigated. All data points represent mean values ± SEM from triplicate determinations. 100% corresponds to 29.6 U/L. (C) Temperature dependency study of cholesterol oxidase activity from C. gleum in 0.11 M MOPS buffer, pH 6.75. The oxidation of cholesterol as a function of temperature is given relative to the highest activity recorded (47.0 U/L) that was taken as 100%. All data points represent mean values ± SEM from triplicate determinations.