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Figure 2 | BMC Biotechnology

Figure 2

From: Cloning, expression and biochemical characterization of the cholesterol oxidase CgChoA from Chryseobacterium gleum

Figure 2

Phylogenetic tree of CgChoA and selected characterized cholesterol oxidases. The unrooted tree was based on the average distance PID and reports the percentage of identity between each protein sequence vs. the consensus sequence generated from the alignment of all the protein sequences considered. In addition to the enzyme CgChoA (subject of this study), the cholesterol oxidases considered are indicated by their PDB identifier and are the (1COY, cofactor non-covalently bound) and the (2I0K, cofactor covalently bound) flavoproteins from Brevibacterium sterolicum, the flavoprotein from Streptomyces sp. SA-COO (1B4V, cofactor non-covalently bound), and the flavoprotein from Chromobacterium sp. DS-1 (3JS8 cofactor covalently bound). The tree was produced with the software Jalview (http://www.jalview.org).

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