Figure 5

Electrophoresis of KmAdhs from K. marxianus GX-UN120. SDS-PAGE analysis on 10% polyacrylamide gel stained with Coomassie light blue. a, KmAdh1; b, KmAdh2; c, KmAdh3; d, KmAdh4. Lane M, protein molecular weight markers (116.0, 66.2, 45.0, 35.0 25.0 and 18.4 kDa). Lane 1, proteins of E. coli Rosetta DE3 harboring the empty plasmid pET-32a(+) in a, c, d and empty pET-30a(+) in b; lane 2, proteins of E. coli Rosetta DE3 harboring the plasmids pET-32a(+)-KmADH1, pET-32a(+)-KmADH3 and pET-32a(+)-KmADH4 in a, c and d and pET-30a(+)-KmADH2 in b; lane 3, the purified recombinant KmAdhs fusion proteins; lane 4, the purified KmAdhs. The recombinant fusion proteins and proteins after digestion with enterokinase light chain were purified with Co–NTA chromatography.