Table 4 Primers used in this study for gene cloning and plasmid construction
Primer name | Sequences (5’ → 3’) | Source |
|---|---|---|
p4hP –pET-28a-S | TGTAGAATTCATGATCTCACCTGCGCA | P. stutzeri |
p4hP –pET-28a-A | ATATAAGCTTCTAGCTGCCGACCAGCTTC | |
p4hP -pEC-XK99E-S | TAATGAATTCGTGAACCCTATGCAAGC | P. stutzeri |
p4hP -pEC-XK99E -A | ATAGTCTAGATCAGAGATACTGTTGCGG | |
p4hB–pET-28a-S | TGATGAATTCCTAGCGCTCGACCAGTTT | B. bronchiseptica |
p4hB –pET-28a-A | CTGCAAGCTTATGATTTCACCTGCTCAGG | |
p4hB -pEC-XK99E-S | TATAGAATTCATGATCTCACCTGCCCAG | B. bronchiseptica |
p4hB -pEC-XK99E -A | TGTCTCTAGATTATTCCACCAGCTTCAG | |
p4hD –pET-28a-S | TATAGAGCTCATGCTGACTCCGACCGA | Dactylosporangium sp. |
p4hD –pET-28a-A | GATCAAGCTTTTAAACTGGCTGGGCAAG | |
p4hD -pEC-XK99E-S | TATAGAGCTCATGCTGACTCCGACCGA | Dactylosporangium sp. |
p4hD -pEC-XK99E-A | GATCTCTAGATTAAACTGGCTGGGCAAG | Â |