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Table 5 Quantitative analysis of six blind samples

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Sample code

Event or gene

GM content (%)

REP1 (%)

REP2 (%)

REP3 (%)

REP4 (%)

Mean value (%)

SD

RSD (%)

Bias (absolute value)

Bias (%)

S1

GHB119

0.5

0.47

0.48

0.51

0.45

0.48

0.02

4.17

-0.02

-3.96

T304-40

5.0

4.86

4.87

5.02

5.03

4.95

0.09

1.82

-0.05

-1.08

Cry2Ae

0.5

0.49

0.50

0.50

0.53

0.51

0.02

3.79

0.01

1.22

S2

GHB119

5.0

4.94

4.99

4.94

5.19

5.02

0.12

2.39

0.02

0.31

T304-40

1.0

1.02

1.07

0.84

0.80

0.93

0.13

13.95

-0.07

-6.84

Cry2Ae

5.0

4.67

5.14

4.85

4.82

4.87

0.20

4.07

-0.13

-2.61

S3

GHB119

2.5

2.46

2.41

2.51

2.50

2.47

0.05

2.02

-0.03

-1.14

T304-40

0.5

0.46

0.49

0.48

0.45

0.47

0.02

4.25

-0.03

-5.86

Cry2Ae

2.5

2.72

2.66

2.64

2.67

2.67

0.03

1.14

0.17

6.93

S4

GHB119

1.0

0.95

1.03

1.02

0.95

0.99

0.04

4.06

-0.01

-1.36

T304-40

2.5

2.46

2.54

2.48

2.56

2.51

0.05

1.99

0.01

0.54

Cry2Ae

1.0

1.06

1.08

1.09

1.09

1.08

0.01

1.22

0.08

8.23

S5

GHB119

0.0

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

T304-40

0.5

0.44

0.44

0.46

0.53

0.47

0.04

8.52

-0.03

-6.10

Cry2Ae

0.0

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

S6

GHB119

0.5

0.47

0.46

0.47

0.48

0.47

0.01

2.13

-0.03

-6.03

T304-40

0.0

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

0.00

Cry2Ae

0.5

0.52

0.51

0.52

0.56

0.53

0.02

4.31

0.03

5.80