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Table 5 Quantitative analysis of six blind samples

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Sample code Event or gene GM content (%) REP1 (%) REP2 (%) REP3 (%) REP4 (%) Mean value (%) SD RSD (%) Bias (absolute value) Bias (%)
S1 GHB119 0.5 0.47 0.48 0.51 0.45 0.48 0.02 4.17 -0.02 -3.96
T304-40 5.0 4.86 4.87 5.02 5.03 4.95 0.09 1.82 -0.05 -1.08
Cry2Ae 0.5 0.49 0.50 0.50 0.53 0.51 0.02 3.79 0.01 1.22
S2 GHB119 5.0 4.94 4.99 4.94 5.19 5.02 0.12 2.39 0.02 0.31
T304-40 1.0 1.02 1.07 0.84 0.80 0.93 0.13 13.95 -0.07 -6.84
Cry2Ae 5.0 4.67 5.14 4.85 4.82 4.87 0.20 4.07 -0.13 -2.61
S3 GHB119 2.5 2.46 2.41 2.51 2.50 2.47 0.05 2.02 -0.03 -1.14
T304-40 0.5 0.46 0.49 0.48 0.45 0.47 0.02 4.25 -0.03 -5.86
Cry2Ae 2.5 2.72 2.66 2.64 2.67 2.67 0.03 1.14 0.17 6.93
S4 GHB119 1.0 0.95 1.03 1.02 0.95 0.99 0.04 4.06 -0.01 -1.36
T304-40 2.5 2.46 2.54 2.48 2.56 2.51 0.05 1.99 0.01 0.54
Cry2Ae 1.0 1.06 1.08 1.09 1.09 1.08 0.01 1.22 0.08 8.23
S5 GHB119 0.0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
T304-40 0.5 0.44 0.44 0.46 0.53 0.47 0.04 8.52 -0.03 -6.10
Cry2Ae 0.0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
S6 GHB119 0.5 0.47 0.46 0.47 0.48 0.47 0.01 2.13 -0.03 -6.03
T304-40 0.0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
Cry2Ae 0.5 0.52 0.51 0.52 0.56 0.53 0.02 4.31 0.03 5.80
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