Table 3 Parameters of standard curves constructed using the quadruplex and simplex PCR assays for GM cotton GHB119, T304-40 and Cry2Ae detection
Target | Linear equation | R2 | PCR efficiency (%) | |
|---|---|---|---|---|
GHB119 | Simplex | y = -3.575x + 43.541 | 0.997 | 90.4 |
Quadruplex | y = -3.524x + 38.780 | 0.998 | 92.2 | |
T304-40 | Simplex | y = -3.549x + 42.421 | 0.993 | 91.3 |
Quadruplex | y = -3.583x + 39.275 | 0.997 | 90.1 | |
Cry2Ae | Simplex | y = -3.516x + 42.034 | 0.998 | 92.5 |
Quadruplex | y = -3.585x + 37.809 | 0.998 | 90.1 | |