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Table 3 Parameters of standard curves constructed using the quadruplex and simplex PCR assays for GM cotton GHB119, T304-40 and Cry2Ae detection

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Target Linear equation R2 PCR efficiency (%)
GHB119 Simplex y = -3.575x + 43.541 0.997 90.4
Quadruplex y = -3.524x + 38.780 0.998 92.2
T304-40 Simplex y = -3.549x + 42.421 0.993 91.3
Quadruplex y = -3.583x + 39.275 0.997 90.1
Cry2Ae Simplex y = -3.516x + 42.034 0.998 92.5
Quadruplex y = -3.585x + 37.809 0.998 90.1
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