Table 2 Primers and probes used for quadruplex real-time PCR assays in this study
Target | Purpose | Primer/probe name | Sequence (5′-3′) | Amplicon length (bp) | Reference |
|---|---|---|---|---|---|
18S rRNA | Eukaryotes gene | 18SrRNA-F | CCTGAGAAACGGCTACCAT | 137 | 19 |
18SrRNA-R | CGTGTCAGGATTGGGTAAT | ||||
18SrRNA-P | FAM-TGCGCGCCTGCTGCCTTCCT –BHQ1 | ||||
ACP1 | Cotton endogenous gene | ACP-FP | ATGAACCAGGGAAGAAGCACC | 97 | This study |
ACP-RP | CCTTATCCACGGTCTCTTGTTTG | ||||
ACP-P | ROX-CATTTACGATGCGTCCAATGCCTG-BHQ2 | ||||
GHB119 | Event-specific detection | GHB119-F1 | AAAACTTTGTGCAGCCTTCG | 130 | This study |
GHB119-R2 | CGCAAACTAGGATAAATTATCGC | ||||
GHB119-P | FAM-TCCCCCTATCTTGCTAAATGGCTCC-BHQ1 | ||||
T304-40 | Event-specific detection | T304-F1 | GTCATTGTAGGGAGTTTGTCCAA | 118 | This study |
T304-R2 | CTGTAGCCACAACACCACTTTG | ||||
T304-P | HEX-TTAATCCCAGTACTCGGCCGTC-BHQ1 | ||||
Cry2Ae | Gene-specific detection | CRY2E-F1 | CTTGCTCTACTTTCCTTCCTCC | 114 | This study |
CRY2E-R2 | CGAAAGACTCAGTTTGCCAGT | ||||
CRY2E-P | CY5-CAAGCCAAGA + C + CTAACGAAAGGAG-BHQ3* |