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Table 1 Mixing method and GM content of six prepared blind samples

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Sample name GM content (%) (w/w) Mixing method
GHB119 T304-40
S1 0.5 5.0 0.2 g A3* + 0.2 g of 10.0% CRM of T304-40
S2 5.0 1.0 0.2 g of 10.0% CRM of GHB119 + 0.2 g of B2*
S3 2.5 0.5 0.2 g of A1* + 0.2 g of B3*
S4 1.0 2.5 0.2 g of A2* + 0.2 g of B1*
S5 0 0.5 0.2 g of non-GM cotton + 0.2 g of B3*
S6 0.5 0 0.2 g of A3* + 0.2 g of non-GM cotton
  1. *A1: 5.0% (w/w) GM cotton GHB119 sample; A2: 2.0% (w/w) GM cotton GHB119 sample; A3: 1.0% (w/w) GM cotton GHB119 sample; B1: 5.0% (w/w) GM cotton T304-40 sample; B2: 2.0% (w/w) GM cotton T304-40 sample; B3: 1.0% (w/w) GM cotton T304-40 sample.
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