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Table 1 Mixing method and GM content of six prepared blind samples

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Sample name

GM content (%) (w/w)

Mixing method

GHB119

T304-40

S1

0.5

5.0

0.2 g A3* + 0.2 g of 10.0% CRM of T304-40

S2

5.0

1.0

0.2 g of 10.0% CRM of GHB119 + 0.2 g of B2*

S3

2.5

0.5

0.2 g of A1* + 0.2 g of B3*

S4

1.0

2.5

0.2 g of A2* + 0.2 g of B1*

S5

0

0.5

0.2 g of non-GM cotton + 0.2 g of B3*

S6

0.5

0

0.2 g of A3* + 0.2 g of non-GM cotton

  1. *A1: 5.0% (w/w) GM cotton GHB119 sample; A2: 2.0% (w/w) GM cotton GHB119 sample; A3: 1.0% (w/w) GM cotton GHB119 sample; B1: 5.0% (w/w) GM cotton T304-40 sample; B2: 2.0% (w/w) GM cotton T304-40 sample; B3: 1.0% (w/w) GM cotton T304-40 sample.
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BMC Biotechnology

ISSN: 1472-6750

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