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Figure 3 | BMC Biotechnology

Figure 3

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Figure 3

Results of sensitivity tests for the established real-time PCR method. (A) Number of positive amplifications out of 20 using four concentrations (10, 5, 2 and 1 copies/μl) of GHB119 or T304-40 ingredient by simplex and quadruplex PCR assays. (B) Mean Ct values and SD for GHB119, T304-40 and Cry2Ae detection via simplex and quadruplex PCR assays using four concentrations (10, 5, 2 and 1 copies/μl) of GHB119 or T304-40 DNA as template. The number above the columns is standard deviations.

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