Figure 3

Results of sensitivity tests for the established real-time PCR method. (A) Number of positive amplifications out of 20 using four concentrations (10, 5, 2 and 1 copies/μl) of GHB119 or T304-40 ingredient by simplex and quadruplex PCR assays. (B) Mean Ct values and SD for GHB119, T304-40 and Cry2Ae detection via simplex and quadruplex PCR assays using four concentrations (10, 5, 2 and 1 copies/μl) of GHB119 or T304-40 DNA as template. The number above the columns is standard deviations.