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Figure 1 | BMC Biotechnology

Figure 1

From: A novel quadruplex real-time PCR method for simultaneous detection of Cry2Aeand two genetically modified cotton events (GHB119 and T304-40)

Figure 1

Schematic diagram of exogenous insertion of GM cotton events T304-40 and GHB119 and alignment of six Cry protein gene sequences. (A–B) Schematic diagram of exogenous insertion of GM cotton events T304-40 (A) and GHB119 (B). LB: left border sequence of T-DNA; 3′mel: terminator from Flaveria bidentis; Cry1Ab: gene encoding Bt endotoxin of Bacillus thuringiensis; Ps7s7: duplicated promoter from subterranean clover stunt virus; P-35S: 35S promoter from cauliflower mosaic virus; Bar: sequence encoding the PAT (phosphinothricin acetyl-transferase) enzyme of Streptomyces hygroscopicus; 3′nos: 3′-terminator of nopaline synthase gene from Agrobacterium tumefaciens; Cry2Ae: gene encoding a Bt endotoxin of Bacillus thuringiensis; T-35S: 35S terminator from cauliflower mosaic virus; P-CsVMV: promoter derived from Cassava vein mosaic virus; RB: right border of transposon from Agrobacterium tumefaciens. Arrows show the primers location developed in this study. (C) DNA fragment sequences of six Cry protein genes showing the locations of the designed primers and probes.

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