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Table 3 DNA oligonucleotides used in this study

From: Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiaeoverexpressing alkaline phosphatase

Primer names

Sequence (5’-3’) restriction sites are italic

Restriction sites

SM16

CCGGAATTCaGACGGGCAGTCbTGTTGGAATAGAAATCAACTATC

EcoRIa, AhdIb

SM17

CATCATTTTATATGTTTATATTCATCTAGAaCCCGGGbGTCGACc TTGATCCTATTACATTATCAATCC

XbaIa, XmaIb, SalIc

SM18

GGATTGATAATGTAATAGGATCAAGTCGACaCCCGGGbTCTAGAcTGAATATAAACATATAAAATGATG

SalIa, XmaIb, XbaIc

SM19

CCCAAGCTTaGACGGGCAGTCbTGAGAAATATGTGAATGTTGAG

HindIIIa, AhdIb

SM28

CGCGGATCCaATGTCTGCATCACACAAGAAGAAGAATGTC

BamHIa

SM29

TTTGCGGCCGCaTCAATCTGATGTGTGTTTGGTGTCCCTAATC

NotIa

Ko419

CGCGTCGACaTTAATTAAAGTCCAATGCTAG

SalIa

Ko420

GATATCGACAAAGGAAAAGGGGCGGCCGCaGGATCCbCTCGAGcTGTATATGAGATAGTTGATTG

NotIa, BamHIb, XhoIc

Ko453

CAATCAACTATCTCATATACACTCGAGaGGATCCbGCGGCC GCcCCCTTTTCCTTTGTCGATATC

XhoIa, BamHIb, NotIc

Ko454

CCCCCCGGGaGCAAATTAAAGCCTTCGAGC

XmaIa

Ko508

CGCGGATCCaATGATGACTCACACATTACCAAGC

BamHIa

Ko509

TTTGCGGCCGCaTCAGTTGGTCAACTCATGGTAGTATTC

NotIa

  1. a,b,cRestriction sites with corresponding restriction enzyme.