Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiaeoverexpressing alkaline phosphatase

Table 2 Growth rate, ATP level, AP activity, ethanol productivity and yield of S. cerevisiae transformants and control strains

Strain	Specific growth rate, g/L/h^*	ATP, μmoles of ATP/mg dry cell weight^**	Alcaline phosphatase activity, nmoles of product/mg of prot.min.^	Ethanol productivity^*		Ethanol yield g/g of consumed glucose^*
Strain	Specific growth rate, g/L/h^*	ATP, μmoles of ATP/mg dry cell weight^**		g/L/h	g/L/g of biomass/h	Ethanol yield g/g of consumed glucose^*
BY4742	0.031 ± 0.002	7.95 ± 0.10	85.2 ± 3.3	0.79 ± 0.014	0.30 ± 0.006	0.379 ± 0.007
BY4742/Pho8vac	0.032 ± 0.001	7.83 ± 0.06	1948.3 ± 175.3	0.92 ± 0.019	0.34 ± 0.007	0.442 ± 0.009
BY4742/Pho8cyt	0.025 ± 0.001	7.56 ± 0.08	1832.4 ± 137.4	0.42 ± 0.004	0.18 ± 0.002	0.202 ± 0.002
AS400		8.93 ± 0.15	45.3 ± 2.5	3.30 ± 0.083		0.396 ± 0.010
AS400/Pho8vac		8.11 ± 0.18	125.3 ± 4.4	3.75 ± 0.075		0.450 ± 0.010

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