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Figure 4 | BMC Biotechnology

Figure 4

From: Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiaeoverexpressing alkaline phosphatase

Figure 4

Linear schemes of plasmids used in this study: pUC57-delta1_2-ADHpr-CYCt-kanMX (A), pUC57-delta1_2-ADHpr-PHO8-CYCt-kanMX (B), pUC57-delta1_2-ADHpr-PHO8_trunc-CYCt-kanMX (C). δ elements are shown as thick black arrows. ADH1 promoter, CYC1 terminator and kanMX4 gene are shown as chessboard-alike, doted and diagonal-hatched boxes, respectively. Unmodified PHO8 gene ORF is shown as white box and truncated PHO8 gene ORF is shown as black doted box. Origin of replication ORI and ampicillin resistance gene (bla) are shown as thin arrows.

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