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Figure 2 | BMC Biotechnology

Figure 2

From: Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiaeoverexpressing alkaline phosphatase

Figure 2

Analysis of expression cassette integration pattern. (A) Southern hybridization. PHO8 gene was used as a probe. HindIII was used for genomic DNA restriction. 1, 12 – plasmid pUC57-delta1_2-ADHpr-PHO8-CYCt-kanMX; 2 – ladder; 3 - wild type strain, BY4742; 4–11 – BY4742/Pho8vac – recombinant strains containing vector pUC57-delta1_2-ADHpr-PHO8-CYCt-kanMX; B) “head-to-tail” conformation of vector integration.

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BMC Biotechnology

ISSN: 1472-6750

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