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Table 2 Cell counts at steady state

From: Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiaeharbouring xylose isomerase

Dilution rate (h-1)

CFU/mL

Pretreatment liquor (%, v/v)

Time (weeks)

Generations

Inoculation

1.8 × 106 ± 0.42 × 106

50

0

-

0.05

3.5 × 107 ± 7.8 × 106

50

1

13

0.1

38 × 106 ± 4.8 × 106

50

2

49

0.1

36 × 106 ± 6.4 × 106

60

4

97

0.125

41 × 106 ± 17 × 106

60

2

61

Total generations

   

220

  1. Steady state biomass concentrations, expressed as plate counts (CFUs/mL) for the mutated strain D5A+ at increasing dilution rates with the corresponding time at which the chemostat was operated at each dilution rate. The biomass concentration at the time of inoculation is shown for comparison. Standard deviations represent triplicate determinations corresponding to measurements at three consecutive residence times.