TY - JOUR AU - Smith, Justin AU - van Rensburg, Eugéne AU - Görgens, Johann F. PY - 2014 DA - 2014/05/15 TI - Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiaeharbouring xylose isomerase JO - BMC Biotechnology SP - 41 VL - 14 IS - 1 AB - Yeasts tolerant to toxic inhibitors from steam-pretreated lignocellulose with xylose co-fermentation capability represent an appealing approach for 2nd generation ethanol production. Whereas rational engineering, mutagenesis and evolutionary engineering are established techniques for either improved xylose utilisation or enhancing yeast tolerance, this report focuses on the simultaneous enhancement of these attributes through mutagenesis and evolutionary engineering of Saccharomyces cerevisiae harbouring xylose isomerase in anoxic chemostat culture using non-detoxified pretreatment liquor from triticale straw. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-14-41 DO - 10.1186/1472-6750-14-41 ID - Smith2014 ER -