Figure 3

Optimal concentration of immobilized enzyme (A) and substrate (B), and time course of the optimized transformation (C) and chiral HPLC analyses of the ( rac )-γ-lactam control (D) and sample after the 9 h transformation (E). The activity of 200 mg/ml immobilized Gla-Linker-Cbd at 60°C (A, 120.6 ± 5.2 μmol/min) and immobilized Gla-Linker-Cbd to 60 mM substrate (B, 338.6 ± 15.2 μmol/min) was set as 100% relative activity. The line in dot in B represents no substrate inhibition to enzyme. C: Substrate concentration 0.6 M, immobilized enzyme concentration 200 mg/ml, transformation temperature 60°C.