Figure 1

Construction of cbd-linker-gla and gla-linker-cbd fusion genes (A) and expression of the two constructs (B) and adsorption isotherm measurements for crude fusion proteins (C). A. his6: his-tag encoding gene; thr and s tag: thrombin cleavage site and S peptide tag on pET30; B. 1.E. coli rosetta (DE3) harboring pET30; 2.E. coli rosetta (DE3) harboring pETcbdgla; 3.E. coli rosetta (DE3) harboring pETglacbd; 4.Purified Cbd-Linker-Gla; 5.Purified Gla-Linker-Cbd C. The total activity of Gla-Linker-Cbd supernatant at 0 h (5500 U) was set as 100% relative activity.