Figure 5From: Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus caseiinhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cellsTranslocation of rCTB–YVAD or rCTB into Caco-2 cells. Caco-2 cells were treated with 50 μM rCTB–YVAD or rCTB for 6 h in the absence or presence of 10 μg/ml LPS. The translocation of rCTB–YVAD or rCTB into Caco-2 cells was detected with immunoblotting using an antibody directed against CT. β-actin was used as the internal control. Data are representative of three separate experiments.Back to article page