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Figure 2 | BMC Biotechnology

Figure 2

From: Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus caseiinhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells

Figure 2

Secretion and GM1-ganglioside-binding activity of rCTB–YVAD. Wild-type L. casei and L. casei transformed with pHIL253, pSCTB–YVAD, or pSCTB were grown at 30°C. The culture supernatants were collected and concentrated 10-fold. Immunoblotting with an antibody directed against CT was performed to analyze the secretion of rCTB–YVAD or rCTB into the supernatant. Data are representative of three separate experiments (A). The GM1-ganglioside-binding activity in the supernatant was analyzed with GM1-ELISA. Data represent the means ± SEM of three separate experiments performed in triplicate (B). **P < 0.01 compared with pHIL253.

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BMC Biotechnology

ISSN: 1472-6750

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