Figure 1From: Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus caseiinhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cellsConstruction of rCTB–YVAD secretion vector pSCTB–YVAD. pSCTB–YVAD includes the promoter of the LDH gene, SS of the β-N-acetylglucosaminidase gene, the CTB–YVAD-coding sequence, His-tag-coding sequence, and the Term of the β-N-acetylglucosaminidase gene in the Lactobacillus–E. coli shuttle vector pHIL253. The nucleotide and amino acid sequences around the CTB/YVAD fusion site are shown.Back to article page