Figure 7

Translation of eYFP mRNAs in BYL and WGE systems in dialysis mode. Reactions were carried out with 3 μg capped mRNA as the template at 25°C and 900 rpm for 18 h and 24 h, respectively. The amount of fluorescent protein (a) was determined by comparison with an eYFP standard curve generated by measuring different eYFP concentrations in BYL translation reactions without a mRNA template. Data represent the averages and standard deviations of six independent translation experiments. (b) SDS-PAGE of the BYL and WGE translation reactions. In each case 10 μl of the translation reactions were loaded on a 4-12% gradient gel. Lane 1: GAA_Omega_eYFP-His in BYL; lane 2: no template control in BYL; lane 3 GAA_Omega_eYFP-His in 5Prime WGE; lane 4: no template control in 5Prime WGE; lane 5: GAA_Omega_eYFP-His in Promega WGE; lane 6: no template control in Promega WGE; M: molecular weight marker.