Figure 6

Translation of firefly and Renilla reniformis mRNAs in the BYL and WGE systems. Reactions were carried out with 2 μg capped mRNA as the template at 25°C and 500 rpm for 18 h. (a) Bar graphs show luciferase activity expressed as relative luciferase units (RLU). Data represent the averages and standard deviations of six independent translation experiments. (b) Typical result of FFLuc translation in the BYL and WGE systems, in which 1 μl of reaction mixture was combined with 50 μl luciferase assay buffer: FFLuc standard with 0.1 μg/μl (1), pIVEX1.3_FFLuc-His in BYL (2), pIVEX1.3_FFLuc-His in WGE (3), no template control in BYL (4), no template control in WGE (5).