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Figure 5 | BMC Biotechnology

Figure 5

From: Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

Figure 5

Translation of eYFP mRNAs with various 5’ and 3’ UTRs in BYL and WGE systems in batch mode. Reactions were carried out with 2 μg capped mRNA as the template at 25°C and 500 rpm for 18 h. The amount of fluorescent protein was calculated by comparing with an eYFP standard curve generated by measuring different eYFP concentrations in BYL translation reactions without the mRNA template. Data represent the averages and standard deviations of six independent translation experiments.

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BMC Biotechnology

ISSN: 1472-6750

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