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Figure 4 | BMC Biotechnology

Figure 4

From: Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

Figure 4

Optimization of reaction conditions in batch mode. Effects on eYFP yield caused by (a) magnesium acetate concentration, (b) potassium acetate concentration, (c) mRNA concentration, (d) presence (+) or absence (-) of poly(A) sequence. Translation reactions were carried out using capped GAA_Omega_eYFP-His mRNA as the template at 25°C and 500 rpm for 18 h. The relative fluorescence intensities are shown. Mean values were calculated from three independent translation experiments.

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BMC Biotechnology

ISSN: 1472-6750

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