Figure 1From: Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation systemPreparation of evacuolated tobacco BY-2 protoplasts by Percoll gradient centrifugation. (a) Evacuolated protoplasts are concentrated at the interface between 40% and 70% Percoll. (b) Representative image of protoplasts before evacuolation (Scale bar = 20 μm). (c) Representative image of evacuolated protoplasts (Scale bar = 20 μm).Back to article page