Figure 5
From: Production of recombinant human annexin V by fed-batch cultivation
Representative dot plots of flow cytometric analysis. Dot plots of B16F10 cells stained with both FITC-labeled annexin V and PI using the BD Biosciences kit or a homemade kit (QuatroG) 12 h after inducing apoptosis with 0 μg/mL, 40 μg/mL or 160 μg/mL of cisplatin. The upper and lower right quadrants of each dot plot represents cells at late (PI and annexin V positive) and early (annexin V positive only) apoptosis.