Figure 4
From: Production of recombinant human annexin V by fed-batch cultivation
Analysis of apoptosis in B16F10 cells. Flow cytometric analysis of apoptotic cells stained with both FITC-labeled annexin V and PI using the BD Biosciences kit or a homemade kit (QuatroG) containing purified rhANXA5 conjugated with FITC. Apoptosis was induced by treating B16F10 cells with 20, 40, 80 or 160 μg/mL of cisplatin and double staining was performed after 6 h (a) or 12 h (b).