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Table 3 Maximum specific growth rates and product yields in anaerobic batch fermentation of 20 g/L glucose and 50 g/L xylose in complex medium (10 g/L yeast extract) using strains TMB3455, TMB3457 and TMB3459

From: Physiological effects of over-expressing compartment-specific components of the protein folding machinery in xylose-fermenting Saccharomyces cerevisiae

  Glucose phase Xylose phase
  TMB3455 (control) TMB3457 (OSM1) TMB3459 (OSM1 ERO1) TMB3455 (control) TMB3457 (OSM1) TMB3459 (OSM1 ERO1)
μ max (1/h) 0.37 ± 0.003 0.38 ± 0.001 0.38 ± 0.003 0.033 ± 0.004 0.024 ± 0.000 0.016 ± 0.008
Yields per substrate (mol/mol sugar)
Y xylt/s 0.14 ± 0.01 0.16 ± 0.01 0.13 ± 0.01
Y glyc/s 0.13 ± 0.01 0.11 ± 0.01 0.11 ± 0.01 0.013 ± 0.001 0.009 ± 0.001 0.009 ± 0.002
Y ac/s 0.030 ± 0.006 0.022 ± 0.000 0.025 ± 0.003 0.022 ± 0.003 0.037 ± 0.005 0.037 ± 0.001
Y etoh/s 2.7 ± 0.6 2.6 ± 0.2 2.6 ± 0.2 1.4 ± 0.1 1.4 ± 0.0 1.1 ± 0.0
Y succ/s 0.005 ± 0.004 0.003 ± 0.001 0.003 ± 0.001 0.003 ± 0.001 0.006 ± 0.000 0.005 ± 0.001
Y x/s (g/mol) 17.5 ± 1.4 18.4 ± 0.9 19.5 ± 0.6 6.4 ± 0.8 6.3 ± 0.7 4.2 ± 0.8
Yields per biomass (mmol/g CDW)
Y xylt/x 22.3 ± 0.8 25.5 ± 1.2 31.0 ± 8.7
Y glyc/x 7.3 ± 0.5 6.4 ± 0.2 6.4 ± 0.0 2.1 ± 0.0 1.5 ± 0.4 2.3 ± 0.9
Y ac/x 1.3 ± 0.4 1.3 ± 0.1 1.5 ± 0.1 3.4 ± 0.0 5.9 ± 0.2 8.8 ± 1.4
Y etoh/x 94.2 ± 5.0 91.3 ± 5.9 93.0 ± 3.5 222 ± 11 228 ± 21 272 ± 62
Y succcxxx 0.12 ± 0.07 0.17 ± 0.08 0.21 ± 0.02 0.54 ± 0.03 0.91 ± 0.03 1.2 ± 0.5
  1. Values are given as mean ± standard deviation of two independent experiments. Data for strains TMB3456 and TMB3458 is reported in Additional file 2: Table S4.