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Table 2 Maximum specific growth rates and product yields in anaerobic batch fermentation of 20 g/L glucose and 50 g/L xylose in defined medium (2X YNB) using strains TMB3455, TMB3457 and TMB3459

From: Physiological effects of over-expressing compartment-specific components of the protein folding machinery in xylose-fermenting Saccharomyces cerevisiae

  Glucose phase Xylose phase
  TMB3455 (control) TMB3457 (OSM1) TMB3459 (OSM1 ERO1) TMB3455 (control) TMB3457 (OSM1) TMB3459 (OSM1 ERO1)
μ max (1/h) 0.29 ± 0.012 0.30 ± 0.019 0.32 ± 0.044 0.029 ± 0.002 0.018 ± 0.001 0.028 ± 0.003
Yields per substrate (mol/mol sugar)
Y xylt/s 0.24 ± 0.03 0.25 ± 0.01 0.20 ± 0.02
Y glyc/s 0.22 ± 0.02 0.20 ± 0.02 0.20 ± 0.05 0.020 ± 0.000 0.016 ± 0.002 0.012 ± 0.001
Y ac/s 0.020 ± 0.001 0.012 ± 0.000 0.017 ± 0.005 0.019 ± 0.001 0.031 ± 0.003 0.029 ± 0.003
Y etoh/s 1.87 ± 0.14 1.96 ± 0.21 1.96 ± 0.37 1.03 ± 0.00 1.24 ± 0.15 0.87 ± 0.00
Y succ/s 0.003 ± 0.000 0.001 ± 0.000 0.002 ± 0.001 0.009 ± 0.000 0.007 ± 0.002 0.006 ± 0.000
Y x/s (g/mol) 10.4 ± 0.5 10.5 ± 0.2 11.9 ± 0.9 3.8 ± 0.4 2.7 ± 0.6 3.9 ± 0.4
Yields per biomass (mmol/g CDW)
Y xylt/x 63.1 ± 0.3 92.9 ± 25.8 50.3 ± 0.8
Y glyc/x 20.2 ± 0.8 18.3 ± 0.5 16.6 ± 0.2 5.2 ± 0.6 5.8 ± 0.7 3.2 ± 0.6
Y ac/x 2.1 ± 0.1 1.8 ± 0.1 1.8 ± 0.0 5.1 ± 0.3 11.7 ± 1.8 7.3 ± 0.0
Y etoh/x 153 ± 8 153 ± 11 147 ± 7 271 ± 30 458 ± 53 223 ± 23
Y succ/x 0.19 ± 0.04 0.28 ± 0.18 0.22 ± 0.04 2.5 ± 0.4 2.4 ± 0.3 1.6 ± 0.22
  1. Values are given as mean ± standard deviation of two independent experiments. Data for strains TMB3456 and TMB3458 is reported in Additional file 2: Table S3.