Table 1 Strains and plasmids used in this study
Name | Relevant genotype | Reference |
|---|---|---|
Plasmids | Â | Â |
p426TEF | URA3 | [32] |
YIplac128 | LEU2 | [33] |
YIplac211 | URA3 | [33] |
YIpDR1 | YIplac128:TDH3p-GXF1-CYC1t | [34] |
YIpDR7 | YIplac211:TDH3p-XYL1(N272D)-ADH1t; PGK1p-XYL2-PGK1t | [35] |
YIpBB1 | YIplac128:TDH3p-FRD1-CYC1t | This study |
YIpBB2 | YIplac128:TDH3p-OSM1-CYC1t | This study |
YIpBB3 | YIplac128:TDH3p-FRD1-CYC1t; TEF1p-ERO1-ERO1t | This study |
YIpBB4 | YIplac128:TDH3p-OSM1-CYC1t; TEF1p-ERO1-ERO1t | This study |
Yeast strains | Â | |
TMB3043 | CEN.PK2-1C; gre3-Δ; his3::PGK1p-XKS1-PGK1t, HIS3; tal1::PGK1p-TAL1-PGK1t; tkl1::PGK1p-TKL1-PGK1t; rki1::PGK1p-RKI1-PGK1t; rpe1::PGK1p-RPE1-PGK1t; ura3, leu2 | [36] |
TMB3452 | TMB3043; ura3::YIpDR7; leu2 | This study |
TMB3455 | TMB3452; leu2::YIplac128 | This study |
TMB3456 | TMB3452; leu2::YIpBB1 | This study |
TMB3457 | TMB3452; leu2::YIpBB2 | This study |
TMB3458 | TMB3452; leu2::YIpBB3 | This study |
TMB3459 | TMB3452; leu2::YIpBB4 | This study |