TLC showing the products resulting from transesterification reactions. T: triolein 65% (substrate); BD: industrial biodiesel (product control); C: transesterification reaction performed with Novozym 435, shown as a control; C-: negative control of a transesterification reaction run without alcohol. Enzymes immobilized on 1) EP100; 2) MP1000; 3) Celite®545. Reaction products of a) LipA; b) LipC; c) LipCmut; d) LipI.3. FAMEs production was clearly detected in lanes 1a, 3a, 1b, 2b, 1c, 2c and C, where spots at the same height of industrial biodiesel appeared.