Figure 4

FACS analysis. The samples were run through the flow cytometer until 10.000 events were collected using no gate. The IgG-T-Rex-XBP-1(s) cells were grown under induction with 0 μg/mL, 0.1 μg/mL, 0.5 μg/mL or 1 μg/mL DOX (on DOX induction, treated (t)) for seven days at 30°C. Then, cells from each group were washed with DOX-free media and grown in DOX-free media for seven more days at 30°C (off DOX induction, washed (w)). The cells from each group were stained with ER-tracker™ green dye, and cell counts vs. ER-Tracker™ signal from IgG-T-REx-XBP-1(s) cells were measured (on DOX induction, treated (t) (A) and off DOX induction, washed (w) (B)). Median fluorescence intensity (MFI) of ER-Tracker™ Green Dye from IgG-T-REx-XBP-1(s) cells (on DOX induction, treated (t) and off DOX induction, washed (w) (C)).