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Figure 3 | BMC Biotechnology

Figure 3

From: Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein

Figure 3

The specific IgG productivity (A), log of viable cell density (B) and western blot analysis of nuclear extracts (C) from the IgG-T-Rex-XBP-1(s) cells grown at 30°C under 0 μg/mL, 0.1 μg/mL, 0.5 μg/mL or 1 μg/mL DOX for seven days (treated, t) and from the same cells seven days after DOX wash-out (washed, w). PL, protein ladder. Error bars represent the standard deviation of the mean of double determination from each of two independent culture samples per group in two independent experiments, n = 4.

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