Diagnostic performance of bladder cancer-associated molecular panel comprised of three biomarkers. ROC curves were plotted to illustrate the performance characteristics of the 3-biomarker signature for the detection of bladder cancer in urine samples using the Q-Plex multiplex assay (solid line) and commercial ELISA assays (dotted line). Based on the area under the ROC curve (AUROC), Youden Index cutoff values that maximized the sum of sensitivity and specificity were determined for the combination of biomarkers. The Q-Plex multiplex assay achieved an overall sensitivity of 0.93 and specificity of 0.81 (AUROC 0.9476). The combination of data from the individual target ELISA assays achieved an overall sensitivity of 0.77 and specificity of 0.91 (AUROC 0.9119). Thus, the comparison of multiplex results with standard ELISA of these three diagnostic biomarkers showed similar results and trends.