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Figure 9 | BMC Biotechnology

Figure 9

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 9

SDS-PAGE analysis of hFasRECD-T-Fc mediated co-immunoprecipitation of the fractionated hFasLECDs cross-linked with SUNBRIGHT ® DE-100MA. Either wild-type hFasRECD-T-Fc (lanes a and c) or hFasRECD-T-Fc (N102Q, N120Q) mutant (lanes b and d) was used for the experiment. Lanes: M, molecular-weight size markers; a and b, peak II (co-immunoprecipitated sample); c and d, peak III (co-immunoprecipitated sample); e, peak II (fractionated sample, 0.3 μg); f, peak III (fractionated sample, 0.3 μg). The components in the peaks II and III are arrowed.

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BMC Biotechnology

ISSN: 1472-6750

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