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Figure 6 | BMC Biotechnology

Figure 6

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 6

Reaction of NFG1CG4-hFasLECD with N -Ethylmaleimide/SUNBRIGHT ® ME-050MA. a) Chemical structures of N-Ethylmaleimide and SUNBRIGHT® ME-050MA. b) SDS-PAGE analysis of the reaction mixtures at each step. Lanes: M, molecular-weight size-markers; a, before reaction; b, after reaction with TCEP; c, PD-10 column “Elution fraction”; d, PD-10 column “Wash fraction”; e, after reaction with N-Ethylmaleimide followed by quenching with L-Cysteine hydrochloride; f, after reaction with SUNBRIGHT® ME-050MA followed by quenching with L-Cysteine hydrochloride. Upper, middle and lower arrows indicate the migration positions of disulfide-bridged subunits, SUNBRIGHT® ME-050MA adduct and non-conjugated subunit/N-Ethylmaleimide adduct of NFG1CG4-hFasLECD, respectively.

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BMC Biotechnology

ISSN: 1472-6750

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