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Figure 10 | BMC Biotechnology

Figure 10

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 10

Cytotoxic activity of N -Ethylmaleimide conjugated NFG1CG4-hFasLECD against HT-29 cells. a) Effect on cell morphology. Panels: upper, 72 h treatment with N-Ethylmaleimide conjugated NFG1CG4-hFasLECD sample (100 ng/ml); lower, 72 h treatment with buffer alone sample. Either treatment was conducted in the presence of anti-FLAG® M2 antibody (2 μg/ml). b) Effect of sample concentration and treatment time. Symbols: white bars, in the absence of the M2 antibody; gray bars, in the presence of the M2 antibody. Standard error of mean under each experimental condition was included as an error bar. *(p < 0.05) and **(p < 0.01) show comparisons with the control experiments using Student’s t-test.

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