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Figure 1 | BMC Biotechnology

Figure 1

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 1

Production of recombinant hFasLECDs in this study. a) Gene structure of expression unit and variations in N-terminal tag sequences. AOX-1 P, P. pastoris alcohol oxidase 1 promoter region; α-Prepro, Saccharomyces cerevisiae α-factor secretion-signal sequence; Tag, tag sequence; hFasLECD (139-281, N184Q, N250Q), human Fas ligand extracellular domain containing deletion mutation from residue 103 to 138 and double substitution mutations (N184Q and N250Q); AOX-1 TT, P. pastoris alcohol oxidase 1 transcription termination region. b) Schematic presentation of P. pastoris cultivation system using disposable plastic bag.

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BMC Biotechnology

ISSN: 1472-6750

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