Skip to main content

Table 1 Purification of the recombinant PelN from E. coli BL21 (DE3) *

From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli

Fraction Activity ** (kU) Protein (mg) Specific activity (U mg−1) Purification (fold) Yield (%)
Crude extract of supernatant 74.2 ± 0.6 109.8 ± 0.9 675.8 ± 55.9 1 100
Ammonium sulphate precipitation 46.6 ± 6.2 39.9 ± 3.9 1,166.5 ± 59.8 1.73 62.75
UNOsphere S column 21.5 ± 0.1 10.5 ± 0.3 2,060.0 ± 71.1 3.05 29.02
  1. *Data represent means ± SD (n = 4) relative to the reference samples;
  2. **The activity was measured on PGA under the standard conditions.