Table 1 Purification of the recombinant PelN from E. coli BL21 (DE3) *
Fraction | Activity ** (kU) | Protein (mg) | Specific activity (U mg−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude extract of supernatant | 74.2 ± 0.6 | 109.8 ± 0.9 | 675.8 ± 55.9 | 1 | 100 |
Ammonium sulphate precipitation | 46.6 ± 6.2 | 39.9 ± 3.9 | 1,166.5 ± 59.8 | 1.73 | 62.75 |
UNOsphere S column | 21.5 ± 0.1 | 10.5 ± 0.3 | 2,060.0 ± 71.1 | 3.05 | 29.02 |