Figure 3From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coliThe recombinant PelN on an SDS-PAGE gel. Lanes: M, molecular standard; 1, supernatant of the crude extract from E. coli BL21 (DE3) harbouring pET22b; 2, supernatant of the crude extract from E. coli BL21 (DE3) harbouring pET-pelN; 3, after ammonium sulphate precipitation and resuspension; and 4, purified recombinant PelN.Back to article page