Figure 1

Multiple sequence alignment of PelN with several Pels of the PL1 family with known structures. Psp62_PelN (Paenibacillus sp. 0602 PelN), Ec16_PelA (Erwinia chrysanthemi EC16 PelA), Bs168_Pel (B. subtilis subsp. subtilis 168 Pel), Bsp47_PL47 (Bacillus sp. TS-47 PL47), Tm_PelA (Thermotoga maritima PelA), and Bsp165_PelA (Bacillus sp. N165 PelA). Numbering begins at the N-terminus of the proteins without signal peptides. Identical and conserved amino acids are highlighted by black and gray, respectively, while gaps are indicated by dashes. Conserved sequence patterns of Pels are denoted by boxes. In the final line, asterisks indicate conserved sites while dots denote semi-conserved sites. Conserved catalytic residues and putative Ca²⁺-binding residues of PelN are denoted by black circle and © symbol, respectively.