Figure 5

Target gene restoration (TGR) of wild-type ply (A) and lytA (B) was only observed in IDM mutants. (I) Full-length target genes were amplified by PCR of samples taken at 3–10 h post-inoculation. Restoration of ply (A, I) and lytA (B, I) was confirmed by PCR using the primer sets ply-P1/ply-P2 and lytA-P1/lytA-P2, respectively, in 3–10-h cultures of IDM-ply/-lytA with and without antibiotic selection. No target gene restoration was observed in IFD-ply/-lytA mutants. (II) Housekeeping gene gki, used as a loading control to evaluate the level of gene expression, was amplified by primer pair gki-up/gki-dn. (III) Fold change refers to ratio of the target gene restoration (ply or lytA) to gki in 3–10-h cultures over the aforementioned ratio found at the earliest time of target gene detection.